Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition

Vaccari, M; Gordon, SN; Fourati, S; Schifanella, L; Liyanage, NP; Cameron, M; Keele, BF; Shen, X; Tomaras, GD; Billings, E; Rao, M; Chung, AW; Dowell, KG; Bailey-Kellogg, C; Brown, EP; Ackerman, ME; Vargas-Inchaustegui, DA; Whitney, S; Doster, MN; Binello, N; Pegu, P; Montefiori, DC; Foulds, K; Quinn, DS; Donaldson, M; Liang, F; Loré, K; Roederer, M; Koup, RA; McDermott, A; Ma, ZM; Miller, CJ; Phan, TB; Forthal, DN; Blackburn, M; Caccuri, F; Bissa, M; Ferrari, G; Kalyanaraman, V; Ferrari, MG; Thompson, D; Robert-Guroff, M; Ratto-Kim, S; Kim, JH; Michael, NL; Phogat, S; Barnett, SW; Tartaglia, J; Venzon, D; Stablein, DM; Alter, G; Sekaly, RP; Franchini, G; Nat. Med.; 2016; 22: 762–770.

Snippet: “…Immediately before use, the biotinylated FcR or C1q was mixed with a 1:4 molar ratio of Streptavadin-PE (ProZyme cat. #PJ31S), and diluted to a final concentration of 1.0 µg/ml FcR/C1q in Luminex Assay Buffer (PBS + 0.1% BSA + 0.05% Tween)...”

Product: Streptavadin-PE

Tolerance is established in polyclonal CD4(+) T cells by distinct mechanisms, according to self-peptide expression patterns

Malhotra, D; Linehan, JL; Dileepan, T; Lee, YJ; Purtha, WE; Lu, JV; Nelson, RW; Fife, BT; Orr, HT; Anderson, MS; Hogquist, KA; Jenkins, MK; Nat. Immunol.; 2016; 17(2): 187-95.

Snippet: “…Monomers were purified and combined with streptavidin-phycoerythrin (PE) or streptavidin-allophycocyanin (APC) (ProZyme) to produce fluorescence-labeled I-Ab tetramers as described…”

Product: Streptavidin-phycoerythrin (PE), streptavidin-allophycocyanin (APC)

Regulated selection of germinal-center cells into the memory B cell compartment

Shinnakasu, R; Inoue, T; Kometani, K; Moriyama, S; Adachi, Y; Nakayama, M; Takahashi, Y; Fukuyama, H; Okada, T; Kurosaki, T; Nature Immunology; 2016; 17(7): 861-869.

Snippet: “…APC (ProZyme) was dialyzed against 3% NaHCO3...”

Product: APC

Hydrophobic CDR3 residues promote the development of self-reactive T cells

Stadinski, BD; Shekhar, K; Gómez-Touriño, I; Jung, J; Sasaki, K; Sewell, AK; Peakman, M; Chakraborty, AK; Huseby, ES; Nat. Immunol.; 2016; 17(8): 946-55.

Snippet: “…anti-CD25 (PC61.5, 25-0251-82), anti-CD90.2 (53-2.1, 17-0902-83), eBioscience; antiCD62L (MEL-14, 104424), anti-CD90.1 (OX-7, 202533) BioLegend; anti-CD8 (5H10, MCD0817) Invitrogen; Streptavidin-RPE (PJRS25)ProZyme...”

Product: Streptavidin-RPE (PJRS25)

T cell receptor cross-reactivity between similar foreign and self peptides influences naive cell population size and autoimmunity

Nelson, RW; Beisang, D; Tubo, NJ; Dileepan, T; Wiesner, DL; Nielsen, K; Wüthrich, M; Klein, BS; Kotov, DI; Spanier, JA; Fife, BT; Moon, JJ; Jenkins, MK; Immunity; 2015; 42(1): 95-107.

Snippet: “…Biotinylated p:I-Ab monomers were used to make tetramers with streptavidin-phycoerythrin (PE) or streptavidin-allophycocyanin (APC) (ProZyme)...”

Product: Streptavidin-allophycocyanin (APC), streptavidin-phycoerythrin (PE)

Interleukin-2-Dependent Allergen-Specific Tissue-Resident Memory Cells Drive Asthma

Hondowicz, BD; An, D; Schenkel, JM; Kim, KS; Steach, HR; Krishnamurty, AT; Keitany, GJ; Garza, EN; Fraser, KA; Moon, JJ; Altemeier, WA; Masopust, D; Pepper, M; Immunity; 2016; 44(1): 155-66.

Snippet: “…Biotinylated I-Ab molecules containing the covalently attached Derp1 114-124 (SNYCQIYPPNV) or Derp1 117-127 (CQIYPPNVNKI) epitope (nonamer core plus two N-terminal flanking amino acids) were made as previously described (Moon et al., 2007). Monomers were tetramerized with streptavidin-PE or streptavidin-APC (ProZyme)...”

Product: Streptavidin-PE, streptavidin-APC

ICOS and Bcl6-dependent pathways maintain a CD4 T cell population with memory-like properties during tuberculosis

Moguche, AO; Shafiani, S; Clemons, C; Larson, RP; Dinh, C; Higdon, LE; Cambier, CJ; Sissons, JR; Gallegos, AM; Fink, PJ; Urdahl, KB; J. Exp. Med.; 2015; 212(5): 715-28.

Snippet: “…Production of the peptide:I-Ab monomeric molecules, and tetramerization of biotinylated peptide:I-Ab with phycoerythrin (PE) or allophycocyanin (APC) fluorophores (ProZyme), was performed as previously described...”

Product: Allophycocyanin (APC) fluorophores

TCR ITAM multiplicity is required for the generation of follicular helper T-cells

Hwang, S; Palin, AC; Li, L; Song, KD; Lee, J; Herz, J; Tubo, N; Chu, H; Pepper, M; Lesourne, R; Zvezdova, E; Pinkhasov, J; Jenkins, MK; McGavern, D; Love, PE; Nat Commun; 2015; 6: 6982.

Snippet: “…then purified and made into tetramers with streptavidin (SA)–phycoerythrin or SA–allophycocyanin (ProZyme , California) as described previously...”

Product: Streptavidin-phycoerythrin, Streptavidin–allophycocyanin

Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment

Spanier, JA; Frederick, DR; Taylor, JJ; Heffernan, JR; Kotov, DI; Martinov, T; Osum, KC; Ruggiero, JL; Rust, BJ; Landry, SJ; Jenkins, MK; McLachlan, JB; Fife, BT; Nat Commun; 2016; 7: 11804.

Snippet: “…Tetramers were produced by incubating p:MHCII monomers with streptavidinAPC (ProZyme # PJ27S) or streptavidin-PE (ProZyme #PJRS27) at a 4:1 molar ratio…”

Product: Streptavidin-APC, streptavidin-PE

Enzyme Micropump‐Based Inhibitor Assays

Ortiz‐Rivera, I; Courtney, TM; A; Sen Advanced Functional Materials; 2016; 26(13): 2135–2142.

Snippet: “…The SAM-modified surfaces were then incubated in a streptavidin (ProZyme) solution (9 × 10 −6 m in 10 × 10 −3 m PBS buffer, pH 7.2) for 3 h, after which the surfaces were washed with 10 × 10 −3 m PBS buffer…”

Product: Streptavidin

N-terminal additions to the WE14 peptide of chromogranin A create strong autoantigen agonists in type 1 diabetes

Jin, N; Wang, Y; Crawford, F; White, J; Marrack, P; Dai, S; Kappler, JW; Proc. Natl. Acad. Sci. U.S.A.; 2015; 112(43): 13318-23.

Snippet: “…Peptides at >98% purity were synthesized and purified by CHI Scientific. The sequence of the ChgA vasostatin-1 peptide (amino acids 11–24, DTKVMKCVLEVISD) (16) was reconfirmed by mass spectrometry at the time of its use in our experiments. Phycoerythrin-streptavidin (PE-SA) was obtained from ProZyme...”

Product: Phycoerythrin-streptavidin (PE-SA)

Dissecting pigment architecture of individual photosynthetic antenna complexes in solution

Wang, Q; Moerner, WE; Proc. Natl. Acad. Sci. U.S.A.; 2015; 112(45): 13880-5.

Snippet: “…APC from Spirulina sp. was purchased from ProZyme...”

Product: APC from Spirulina sp.

Convective flow reversal in self-powered enzyme micropumps

Ortiz-Rivera, I; Shum, H; Agrawal, A; Sen, A; Balazs, AC; Proc. Natl. Acad. Sci. U.S.A.; 2016; 113(10): 2585-90.

Snippet: “…The SAM-modified surfaces were then incubated in a streptavidin (ProZyme) solution (9 μM in 10 mM PBS) for 3 h, after which the surfaces were washed with 10 mM PBS buffer...”

Product: Streptavidin

Light-activated RNA interference in human embryonic stem cells

Huang, X; Hu, Q; Braun, GB; Pallaoro, A; Morales, DP; Zasadzinski, J; Clegg, DO; Reich, NO; Biomaterials; 2015; 63: 70-9.

Snippet: “… Streptavidin (ProZyme) was then coated on biotinylated oligonucleotides on HGN to bridge between nucleic acid and biotinylated TAT-peptide, by adding at 1 mg/mL to ∼0.05 nM nanoparticle in the presence of 0.5 × PBST (DPBS with 0.1% Tween-20) and incubating at RT …”

Product: Streptavidin

TCR sequencing of single cells reactive to DQ2.5-glia-α2 and DQ2.5-glia-ω2 reveals clonal expansion and epitope-specific V-gene usage

Dahal-Koirala, S; Risnes, LF; Christophersen, A; Sarna, VK; Lundin, KE; Sollid, LM; Qiao, SW; Mucosal Immunol; 2016; 9(3): 587-96.

Snippet: “… DQ2.5-glia-α2 (PQPELPYPQPE, underlined 9-mer core amino acid sequence) and DQ2.5-glia-ω2 (FPQPEQPFPWQP; Table 1) were multimerized on phycoerythrin-labeled streptavidin (Invitrogen, Waltham, MA) or allophycocyanin-labeled streptavidin (ProZyme, Hayward, California…”

Product: Allophycocyanin-labeled streptavidin

Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells

Traenkle, B; Emele, F; Anton, R; Poetz, O; Haeussler, RS; Maier, J; Kaiser, PD; Scholz, AM; Nueske, S; Buchfellner, A; Romer, T; Rothbauer, U; Mol. Cell Proteomics; 2015; 14(3): 707-23.

Snippet: “… The biotinylated Nbs were applied at a concentration of 1 μg/ml. Peptide-bound Nbs were detected with 2.5 μg/ml streptavidin-phycoerythrin (PE) solution (ProZyme, Hayward , California) dissolved in 1×Roche buffer 0.05% Tween. …”

Product: Streptavidin-phycoerythrin (PE)

Gut Microbial Membership Modulates CD4 T Cell Reconstitution and Function after Sepsis

Cabrera-Perez, J; Babcock, JC; Dileepan, T; Murphy, KA; Kucaba, TA; Badovinac, VP; Griffith, TS; J. Immunol.; 2016; 197(5):1692-8.

Snippet: “…Biotinylated monomers were made into tetramers with streptavidin– PE or streptavidin–allophycocyanin (ProZyme, Hayward, California) as previously described (12, 14). The enriched cells were then stained with fluorochrome- labeled mAb to detect additional surface …”

Product: Streptavidin-PE, Streptavidin-allophycocyanin

Effect of thymic stimulation of CD4+ T cell expansion on disease onset and progression in mutant SOD1 mice

Sheean, RK; Weston, RH; Perera, ND; D'Amico, A; Nutt, SL; Turner, BJ; J Neuroinflammation; 2015; 12: 40.

Snippet: “… Cells were transferred to glass conical tubes and centrifuged at 1,700 rpm for 7 min and incubated with 20 μl of an antibody cocktail of rat CD4-FITC (Molecular Probes), CD8-APC (ProZyme, Hayward, California), B220-Alexa 680 (ProZyme), GR-1-PeCy7 and M1-70-Pe on ice for …”

Product: CD8-APC

Tricyclic covalent inhibitors selectively target Jak3 through an active site thiol

Goedken, ER; Argiriadi, MA; Banach, DL; Fiamengo, BA; Foley, SE; Frank, KE; George, JS; Harris, CM; Hobson, AD; Ihle, DC; Marcotte, D; Merta, PJ; Michalak, ME; Murdock, SE; Tomlinson, MJ; Voss, JW; J. Biol. Chem.; 2015; 290(8): 4573-89.

Snippet: “… phosphotyrosine antibody, Cat. no. 61T66KLB, Cisbio, Bedford, MA), and 3.12 μg/ml SAXL (Phycolink streptavidin-allophycocyanin acceptor, Cat. no. PJ25S, ProZyme, California)). The developed reaction was …”

Product: SAXL (Phycolink streptavidin-allophycocyanin acceptor, catalog no. PJ25S)

Phenotypic and functional characterization of herpes simplex virus glycoprotein B epitope-specific effector and memory CD8+ T cells from symptomatic and asymptomatic individuals with ocular herpes

Khan, AA; Srivastava, R; Spencer, D; Garg, S; Fremgen, D; Vahed, H; Lopes, PP; Pham, TT; Hewett, C; Kuang, J; Ong, N; Huang, L; Scarfone, VM; Nesburn, AB; Wechsler, SL; BenMohamed, L; J. Virol.; 2015; 89(7): 3776-92.

Snippet: “… The gB tetramers were prepared and provided by the NIH Tetramer Core Facility, Emory University, Atlanta, GA. Briefly, tetramers, were linked to streptavidin-PE (PJRS25; ProZyme) and adjusted to a concentration of 1 mg/ml. …”

Product: Streptavidin-PE

Transcriptional regulation of the daptomycin gene cluster in Streptomyces roseosporus by an autoregulator, AtrA

Mao, XM; Luo, S; Zhou, RC; Wang, F; Yu, P; Sun, N; Chen, XX; Tang, Y; Li, YQ; J. Biol. Chem.; 2015; 290(12): 7992-8001.

Snippet: “… About 500 ng of biotinylated DNA was incubated with streptavidin-agarose (ProZyme) and washed twice with the binding buffer (20 mm Tris, pH 8.0, 50 mm NaCl, 1 mm EDTA, and 10% glycerol) to eliminate the unbound probes and save the pellet. …”

Product: Streptavidin-agarose

Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull

Rodgers, ML; Paulson, J; Hoskins, AA; RNA; 2015; 21(5): 1031-41.

Snippet: “… England Biolabs). Determination of the extent of Biotinylation of the SNAP AP tag. Streptavidin (50 µg in 5 µL; ProZyme) was incubated with 20–40 µL of labeled yeast whole-cell extract for 10 min at room temperature. Samples …”

Product: Streptavidin

Serine 302 Phosphorylation of Mouse Insulin Receptor Substrate 1 (IRS1) Is Dispensable for Normal Insulin Signaling and Feedback Regulation by Hepatic S6 Kinase

Copps, KD; Hançer, NJ; Qiu, W; White, MF; J. Biol. Chem.; 2016; 291(16): 8602-17.

Snippet: “… a rotary plate shaker (80 rpm). After removal of the biotinylated detection antibody, the beads were incubated with shaking in 25 μl of 1 μg/ml streptavidin-phycoerythrin (ProZyme) for 15 min. All solutions were then removed, and …”

Product: Streptavidin-phycoerythrin

Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development

Nanton, MR; Lee, SJ; Atif, SM; Nuccio, SP; Taylor, JJ; Bäumler, AJ; Way, SS; McSorley, SJ; Eur. J. Immunol.; 2015; 45(2): 428-41.

Snippet: “…The molaramount of biotinylated OVA was measured via Western blotby incubating a fixed amount of biotinylated OVA with differ-ing amounts of SA-PE (ProZyme) at room temperature, followedby Native PAGE (Bio-Rad Laboratories)...”

Product: Streptavidin-PE

NOD mice are functionally deficient in the capacity of cross-presentation

Lee, CN; Lew, AM; Shortman, K; Wu, L; Immunol. Cell Biol.; 2015; 93(6): 548-57.

Snippet: “… Each purified monoclonal antibody was conjugated to either biotin (Invitrogen/Molecular Probes, Eugene, OR), allophycocyanin (ProZyme, Hayward, California), R-phycoerythrin (PE), fluorescein-5-isothiocyanate, Alexa Fluor 594 (Alex594), Alexa Fluor 680 (Alex680 …”

Product: Allophycocyanin

Soluble endothelial cell selective adhesion molecule and cardiovascular outcomes in patients with stable coronary disease: A report from the Heart and Soul Study

Park, M; Kulkarni, A; Beatty, A; Ganz, P; Regan, M; Vittinghoff, E; Whooley, M; Atherosclerosis; 2015; 243(2): 546-52.

Snippet: “… Fluorescent signals were generated using Streptavidin-R-Phycoerythrin (SA-RPE: ProZyme PJ31S) and read on a Luminex LX200 reader. An 8-point calibration curve was made gravimetrically by spiking each antigen into a calibration matrix. …”

Product: Streptavidin-R-Phycoerythrin

Structural Analysis of Multi-Helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA

Cornilescu, G; Didychuk, AL; Rodgers, ML; Michael, LA; Burke, JE; Montemayor, EJ; Hoskins, AA; Butcher, SE; J. Mol. Biol.; 2016; 428(5 Pt A): 777-89.

Snippet: “…Streptavidin (ProZyme) was then added to the slide and incubated, and unbound proteins were removed by copious washing with PBS...”

Product: Streptavidin

Fiber composite slices for multiplexed immunoassays

Kim, J; Bae, S; Song, S; Chung, K; Kwon, S; Biomicrofluidics; 2015; 9(4): 044109.

Snippet: “… and 13-7179-85. Alkaline phosphatase substrate (alka- line phosphatase blue) was purchased from Sigma Aldrich. Streptavidin-linked phycoerythrin (SA-PE) was purchased from ProZyme. [2-(N-morpholino)ethanesulfonic …”

Product: Streptavidin-linked phycoerythrin (SA-PE)

Characterization of a New Epitope of IRBP That Induces Moderate to Severe Uveoretinitis in Mice With H-2b Haplotype

Mattapallil, MJ; Silver, PB; Cortes, LM; Leger, AJ; Jittayasothorn, Y; Kielczewski, J; Moon, JJ; Chan, CC; Caspi, RR; Investigative Ophthalmology & Visual Science; 2015; 56(9): 5439-49.

Snippet: “… Tetramer Staining of hIRBP651-670–Specific Cells. The IA b -hIRBP654-664 tetramer reagent labeled with streptavidin-phycoerythrin (PE) or streptavidin-allophycocyanin (APC; ProZyme, Hayward, California) was generated by Moon et al., 11 using a method described …”

Product: Streptavidin-phycoerythrin (PE), streptavidin-allophycocyanin

Small molecule mediated inhibition of RORγ-dependent gene expression and autoimmune disease pathology in vivo

Banerjee, D; Zhao, L; Wu, L; Palanichamy, A; Ergun, A; Peng, L; Quigley, C; Hamann, S; Dunstan, R; Cullen, P; Allaire, N; Guertin, K; Wang, T; Chao, J; Loh, C; Fontenot, JD; Immunology; 2016; 147(4): 399-413.

Snippet: “…Europium-labeled antibody to detect GST was from Perkin Elmer and Streptavidin- APC was from ProZyme. The ability of the ligand to enhance (agonists) or inhibit (inverse agonists) the interaction between RORγ and a TRAP220 co-activator peptide was assayed …”

Product: Streptavidin- APC

Calling Biomarkers in Milk Using a Protein Microarray on Your Smartphone

Ludwig, SK; Tokarski, C; Lang, SN; van Ginkel, LA; Zhu, H; Ozcan, A; Nielen, MW; PLoS ONE; 2015; 10(8): e0134360.

Snippet: “… USA). Streptavidin-conjugated R-phycoerythrin was bought from ProZyme, California and color-encoded carboxylated SeroMAP microspheres (microsphere sets 050 and 067) from Luminex (Austin, TX). …”

Product: Streptavidin-conjugated R-phycoerythrin

Technical decision making with higher order structure data: utilization of differential scanning calorimetry to elucidate critical protein structural changes resulting from oxidation

Arthur, KK; Dinh, N; Gabrielson, JP; J Pharm Sci; 2015; 104(4): 1548-54.

Snippet: “… inhibition assay, the antigen was labeled with Europium 3+ chelate(E 3+ ) and the receptor was a soluble fusion protein with a FLAG peptide and Fc moiety secondarily labeled with an anti-FLAG antibody conjugated to an allophycocyanin (APC) fluorochrome (ProZyme PJ255). …”

Product: Allophycocyanin (APC)

Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species

Silbereisen, A; Tamborrini, M; Wittwer, M; Schürch, N; Pluschke, G; BMC Microbiol.; 2015; 15(1): 198.

Snippet: “… with PBS containing 0.05 % Tween-20 and the beads were resuspended in 50 μL of biotinylated detection antibody at a concentration of 10 μg/mL in blocking buffer and incubated for 1 h. After repeated washing, 50 μL of a streptavidin-R phycoerythrin (ProZyme Inc.) solution …”

Product: Streptavidin-R phycoerythrin

Differentiation of classical swine fever virus infection from CP7_E2alf marker vaccination by a multiplex microsphere immunoassay

Xia, H; Harimoorthy, R; Vijayaraghavan, B; Blome, S; Widén, F; Beer, M; Belák, S; Liu, L; Clin. Vaccine Immunol.; 2015; 22(1): 65-71.

Snippet: “… Following the same washing step as before, 50 μl of 10-μg/ml streptavidin-R-phycoerythrin conjugate (ProZyme, Hayward, California) in PBS with 1% BSA was added to each well and incubated at 37°C for 30 min on a plate shaker at 300 rpm. …”

Product: Streptavidin-R-phycoerythrin conjugate

Structure activity optimization of 6H-pyrrolo[2,3-e][1,2,4]triazolo[4,3-a]pyrazines as Jak1 kinase inhibitors

Friedman, M; Frank, KE; Aguirre, A; Argiriadi, MA; Davis, H; Edmunds, JJ; George, DM; George, JS; Goedken, E; Fiamengo, B; Hyland, D; Li, B; Murtaza, A; Morytko, M; Somal, G; Stewart, K; Tarcsa, E; Van Epps, S; Voss, J; Wang, L; Woller, K; Wishart, N; Bioorg. Med. Chem. Lett.; 2015; 25(20): 4399-404.

Snippet: “… After 60 min, the enzymatic reaction was quenched by addition of EDTA (to final concentration 100 mM) and developed by addition of PT66-K (europium labeled antiphosphotyrosine antibody cat #61T66KLB Cisbio, Bedford, MA) and 3.12 lg/mL phycolink streptavidin-allophycocyanin acceptor (cat #PJ25S, ProZyme, California). …”

Product: Streptavidin-allophycocyanin (cat #PJ25S)

Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA

Glushakova, LG; Sharma, N; Hoshika, S; Bradley, AC; Bradley, KM; Yang, Z; Benner, SA; Anal. Biochem.; 2015; 489: 62-72.

Snippet: “… was performed accordingly to the direct hybridization protocol (DHA) provided by Luminex: 95 °C for 5 min, cool to 56 °C at a speed of 0.1 °C/s, and 15 min at 56 °C. The Tm buffer (25 μl of 1×) contained streptavidin-R-phycoerythrin (2 μg, PJRS14, ProZyme, Hayward, California …”

Product: Streptavidin-R-phycoerythrin

Self-assembly formed by a short DNA probe pair: Application for highly sensitive mRNA species detection without reverse transcription

Usui, M; Fujikawa, T; Osawa, M; Hakii, C; Ikumi, N; Nozaki, T; Kitamura, N; Hatta, Y; Fujiwara, S; Takei, M; Biochem. Biophys. Res. Commun.; 2015; 467(4): 1012-8.

Snippet: “…Thereafter, 50 μl streptavidin–phycoerythrin (5 ng/μl) (ProZyme, California) was added to each well, and the plate was shaken on a plate mixer for approximately 20 s and then left in the dark at room temperature for 10 min. …”

Product: Streptavidin–phycoerythrin

High-throughput multiplexed xMAP Luminex array panel for detection of twenty two medically important mosquito-borne arboviruses based on innovations in synthetic biology

Glushakova, LG; Bradley, A; Bradley, KM; Alto, BW; Hoshika, S; Hutter, D; Sharma, N; Yang, Z; Kim, MJ; Benner, SA; J. Virol. Methods; 2015; 214: 60-74.

Snippet: “…Tm buffer (25 μL of 1X) containing streptavidin-R-phycoerythrin (2 μg, PJRS14, ProZyme, Hayward, California) were added to the each hybridization mixture, which was then incubated at 55 °C for 5 min...”

Product: Streptavidin-R-phycoerythrin

The use of anti-Müllerian hormone as diagnostic for gonadectomy status in dogs

Themmen, AP; Kalra, B; Visser, JA; Kumar, A; Savjani, G; de Gier, J; Jaques, S; Theriogenology; 2016; 86(6):1467-74.

Snippet: “… Next, the wells were washed and 100 μL of streptavidin labelled horseradish peroxidase enzyme conjugate (ProZyme, Hayward, California cat # CJ30H) was added to each well and subsequently incubated with shaking for an additional 30 minutes at room temperature and …”

Product: Streptavidin labelled horseradish peroxidase enzyme conjugate (# CJ30H)

Recognition of microbial glycans by human intelectin-1

Wesener, DA; Wangkanont, K; McBride, R; Song, X; Kraft, MB; Hodges, HL; Zarling, LC; Splain, RA; Smith, DF; Cummings, RD; Paulson, JC; Forest, KT; Kiessling, LL; Nat. Struct. Mol. Biol.; 2015; 22(8): 603-10.

Snippet: “…To fabricate carbohydrate-displaying surfaces, 0.5 µg of streptavidin (ProZyme, Cat. no. SA20) was adsorbed onto a Maxisorp (Nunc) flat bottom 96 well plate in phosphate-buffered saline (PBS)...”

Product: Streptavidin

Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection

Wiesner, DL; Specht, CA; Lee, CK; Smith, KD; Mukaremera, L; Lee, ST; Lee, CG; Elias, JA; Nielsen, JN; Boulware, DR; Bohjanen, PR; Jenkins, MK; Levitz, SM; Nielsen, K; PLoS Pathog.; 2015; 11(3): e1004701.

Snippet: “…Streptavidin-Phycoerythrin (ProZyme) was added to pMHCII monomers at a 4:1 molar ratio...”

Product: Streptavidin-Phycoerythrin

A Numerically Subdominant CD8 T Cell Response to Matrix Protein of Respiratory Syncytial Virus Controls Infection with Limited Immunopathology

Liu, J; Haddad, EK; Marceau, J; Morabito, KM; Rao, SS; Filali-Mouhim, A; Sekaly, RP; Graham, BS; PLoS Pathog.; 2016; 12(3): e1005486.

Snippet: “…Streptavidin (SA)-PE was purchased from ProZyme, Hayward, California. Violet fluorescent reactive dye (Invitrogen, California), anti-CD19- and anti-CD16/32-Pacific blue (eBioscience) were used as cell viability and lineage markers to exclude dead and non-T cells from analysis...”

Product: Streptavidin-PE

General Approach for Tetramer-Based Identification of Autoantigen-Reactive B Cells: Characterization of La-and snRNP-Reactive B Cells in Autoimmune BXD2 Mice

Hamilton, JA; Li, J; Wu, Q; Yang, PA; Luo, B; Li, H; Bradley, JE; Taylor, JJ; Randall, TD; Mountz, JD; Hsu, HC; The Journal of Immunology; 2015; 194(10): 5022-5034.

Snippet: “… in DMSO to a stock concentration of 10 mM and diluted to 1 mM in pure dH 2 O. Suspended peptides were aliquoted and stored at −80°C. Tetramers were generated by adding biotinylated peptide step-wise in 1/10 volumes to 6.7 μM SA–Rhodophyta-PE (ProZyme) at a molar …”

Product: Streptavidin–Rhodophyta-PE

Igs as substrates for transglutaminase 2: implications for autoantibody production in celiac disease

Iversen, R; du Pré, MF; Di Niro, R; Sollid LM; The Journal of Immunology; 2015; 195(11): 5159-5168.

Snippet: “… Binding to TG2 was assessed using TG2 multimers prepared by preincubation of biotinylated BirA-TG2 (final concentration 5 μg/ml) with allophycocyanin-conjugated streptavidin (ProZyme) at a 6:1 molar ratio in 2% FCS/PBS for 3 h. The murine T cell hybridoma line BW58α − β …”

Product: Allophycocyanin-conjugated streptavidin

Development of a multiplex fluorescent microsphere immunoassay for diagnosis of the porcine disease complex

Ransburgh, R; Thesis; 2015.

Snippet: “… same method previously described. The fluorescent reporter, Streptavidin-R-Phycoerythrin (ProZyme) was diluted to 2.5µg/ml in PBS-BN and 50µl was added to each well. The plates were incubated by shaking for 30 minutes at room temperature. …”

Product: Streptavidin-R-Phycoerythrin

Phosphorylated α-synuclein in Parkinson's disease: correlation depends on disease severity

Stewart, T; Sossi, V; Aasly, JO; Wszolek, ZK; Uitti, RJ; Hasegawa, K; Yokoyama, T; Zabetian, CP; Leverenz, JB; Stoessl, AJ; Wang, Y; Ginghina, C; Liu, C; Cain, KC; Auinger, P; Kang, UJ; Jensen, PH; Shi, M; Zhang, J; Acta Neuropathol Commun; 2015; 3: 7.

Snippet: “… For each subject, 75 μl CSF was diluted with 25 μl assay diluent (0 · 1% bovine serum albumin [Sigma, St Louis, MO] in PBS). Analytes were detected using biotinylated anti-human pS129 antibody [27] and streptavidin-R-PE (ProZyme, Hayward, California). …”

Product: Streptavidin-R-PE

Multiplex detection of plant pathogens through the Luminex MagPlex bead system

van der Vlugt, RA; van Raaij, H; de Weerdt, M; Bergervoet, JH; Methods Mol. Biol.; 2015; 1302: 283-99.

Snippet: “… 2nd biotinylated antibodies (SAS). Streptavidin-R-Phycoerythrin (ProZyme)...”

Product: Streptavidin-R-Phycoerythrin

The Regulation and Consequences of Type-2 Helper T Cells During Pulmonary Cryptococcal Infection

Wiesner, DL; Thesis; 2015.

Snippet: “…Streptavidin-Phycoerythrin (ProZyme) was added to pMHCII monomers at a 4:1 molar ratio...”

Product: Streptavidin-Phycoerythrin

Cutting edge: Bcl6-interacting corepressor contributes to germinal center T follicular helper cell formation and B cell helper function

Yang, JA; Tubo, NJ; Gearhart, MD; Bardwell VJ; Jenkins MK; The Journal of Immunology; 2015; 194(12): 5604-8.

Snippet: “… The conjugate was formed by mixing biotinylated 2W peptide (GenScript) with streptavidin-PE (ProZyme) at a 4:1 ratio. Cell enrichment and flow cytometry. Enrichment was performed as previously described by mixing cell suspensions with 1 μg PE (ProZyme) …”

Product: Streptavidin-PE, PE

Cross-platform detection of epigenetic modifications from extracted chromatin in leucocytes from blood

Zhou, Z; Cho, IIH; Shan, Z; Irudayaraj, J; Analytical Chemistry Research; 2015; 4: 39-44.

Snippet: “… Louis, MO). Rabbit anti-5hmC antibody was supplied by Active Motif (Carlsbad, California). Streptavidin was purchased from ProZyme, Hayward, California. 2% Protease Inhibitor cocktail was purchased from New England Biolabs. 2.2. Chromatin extraction from frozen blood. …”

Product: Streptavidin

HTRF Kinase Assay Development and Methods in Inhibitor Characterization

Jia, Y; Manuia, M; Juarez, J; Methods Mol. Biol.; 2016; 1360: 1-18

Snippet: “… Phycolink™ Streptavidin- XL Allophycocyanin Conjugate” (ProZyme): 2 mg/ml in buffer containing 10 mM Tris–HCl, pH 8.2, 150 mM NaCl. Aliquots were stored at 4 °C…”

Product: Streptavidin Allophycocyanin Conjugate